TABLE 4.

Comparison of ELISA and DIA

MAb (epitope)a	Assayb	Assay result with Akabane virus isolateb
		OBE-1	JaGAr39	Iriki	KS-1/E/85	KSB-2/C/87	KSB-3/P/90	ON-1/P/93
13A9 (E)	ELISA	1,024	<1	<1	<1	<1	<1	<1
	DIA	0.54	0.02	0.02	0.01	0.00	0.03	0.02
15H6 (D)	ELISA	1,024	256	16	1	64	16	<1
	DIA	0.54	0.68	0.10	0.05	0.51	0.36	0.03
12H6 (C2)	ELISA	1,024	256	<1	<1	4	<1	<1
	DIA	0.38	0.52	0.14	0.05	0.13	0.19	0.06
11C4 (C1)	ELISA	1,024	256	256	16	16	64	4
	DIA	0.54	0.65	0.49	0.13	0.22	0.46	0.28
19B4 (B)	ELISA	1,024	256	1,024	64	64	256	16
	DIA	0.48	0.59	0.40	0.18	0.46	0.41	0.24
13H9 (A2)	ELISA	1,024	<1	1,024	256	64	1,024	<1
	DIA	0.46	0.50	0.40	0.19	0.45	0.37	0.03
5B6 (A1)	ELISA	4,096	1,024	4,096	256	1,024	1,024	256
	DIA	0.36	0.44	0.31	0.12	0.34	0.24	0.20

^aEpitopes were determined by competitive binding assays. 

^bELISA results are given as titers. DIA results are absorbances.