TABLE 2.
Estimation of concentrations of antibody to individual pneumococcal types in reference serum lot 89SF by cross-standardization by the mHSA comix methoda
| Type used as reference standard | Concn (μg/ml) of IgG antibody in serum 89SF against the following type PSs:
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 3 | 4 | 5 | 6B | 7F | 9V | 14 | 18C | 19F | 23F | |
| 1 | 7.6 | 5.8 | 3.5 | 14.8 | 5.3 | 8.3 | 22.9 | 6.8 | 6.7 | 10.6 | |
| 3 | 2.0 | 1.7 | 1.1 | 4.4 | 1.7 | 2.6 | 6.9 | 2.0 | 2.1 | 3.5 | |
| 4 | 4.7 | 5.6 | 2.6 | 10.0 | 4.1 | 6.1 | 16.7 | 4.8 | 4.9 | 8.8 | |
| 5 | 11.0 | 13.0 | 10.1 | 23.8 | 9.8 | 13.8 | 40.0 | 11.3 | 11.7 | 17.9 | |
| 6B | 8.0 | 9.7 | 7.2 | 4.4 | 6.9 | 10.5 | 28.4 | 8.2 | 8.5 | 13.9 | |
| 7F | 6.2 | 7.8 | 5.4 | 3.5 | 13.2 | 8.9 | 20.9 | 6.2 | 6.4 | 10.6 | |
| 9V | 5.6 | 7.2 | 4.9 | 3.2 | 12.0 | 4.7 | 18.9 | 5.7 | 5.8 | 9.6 | |
| 14 | 8.2 | 10.4 | 7.2 | 6.3 | 17.7 | 6.9 | 10.3 | 8.3 | 8.5 | 14.1 | |
| 18C | 5.1 | 6.6 | 4.3 | 3.0 | 10.9 | 4.1 | 7.7 | 16.5 | 5.2 | 8.4 | |
| 19F | 7.3 | 11.9 | 7.7 | 5.1 | 22.7 | 8.4 | 13.6 | 35.4 | 11.0 | 18.8 | |
| 23F | 5.3 | 7.0 | 4.5 | 3.1 | 10.8 | 4.3 | 8.1 | 17.3 | 5.4 | 5.5 | |
| Meanb | 6.2 | 7.7 | 5.5 | 3.7 | 12.8 | 5.2 | 8.5 | 20.2 | 6.5 | 6.4 | 10.8 |
a
Data are for one of six assays to estimate IgG antibodies. Similar assays were done to estimate levels of total antibody to each type. Reference antibody values to a given pneumococcal type were used.
b
Arithmetic means of antibody estimates obtained with individual types with their previously assigned IgG antibody concentrations (14) to generate the reference curve. Estimates obtained with types 3, 5, and 19F as standards were excluded from the mean calculations.