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Flow cytometry for the assay of C3 deposition on BCG. Bacilli were incubated with affinity-purified rabbit anti-LAM IgG or normal rabbit Ig, washed twice, and exposed to a dilution of B-depleted (dpl) serum with or without EGTA for 1 h at 37°C and then washed again. The conjugate was FITC-labeled anti-C3c. The values for median channel fluorescence intensity in the histograms from top to bottom were 14.33, 3.28, and 9.31.
