FIG. 4.

Immunoblot analysis of sera of FIV-RBC-immunized and control cats at the time of challenge. Purified FIV was subjected to SDS-PAGE under reducing conditions, blotted onto nitrocellulose, and reacted with sera from cats immunized with FIV-RBC (lanes A to D), with sera from FIV-infected cats (lanes E and F), with one serum from a BSA-RBC-immunized cat (lane G), and with an SUgp-specific (lane H) or control (lane I) mouse serum. Lane L was stained for glycoproteins by the ECL glycoprotein detection system in accordance with manufacturer’s instructions.