Table 3.

Analysis of variance of regression model.

Source	df	Chrysophanol				Aurantio-obtusin				Membrane flux
		Sum of square	Mean square	F-value	P-value	Sum of Square	Mean Square	F-value	P-value	Sum of square	Mean square	F-value	P-value
Model	9	372.69	41.41	43.85	<0.0001*	319.67	35.52	69.51	<0.0001*	215.69	23.97	162.08	<0.0001*
X1	1	169.00	169.00	178.95	<0.0001*	145.69	145.69	285.12	<0.0001*	42.32	42.32	286.22	<0.0001*
X2	1	27.83	27.83	29.46	0.0010*	30.23	30.23	59.15	0.0001*	9.68	9.68	65.47	<0.0001*
X3	1	60.34	60.34	63.89	<0.0001*	45.65	45.65	89.34	<0.0001*	124.82	124.82	844.19	<0.0001*
X1X2	1	5.43	5.43	5.75	0.0476*	5.13	5.13	10.04	0.0157*	0.72	0.72	4.89	0.0627
X1X3	1	41.93	41.93	44.39	0.0003*	49.91	49.91	97.68	<0.0001*	10.56	10.56	71.44	<0.0001*
X2X3	1	1.06	1.06	1.12	0.3244	1.28	1.28	2.50	0.1579	1.32	1.32	8.94	0.0202*
X12	1	20.36	20.36	21.55	0.0024*	9.09	9.09	17.79	0.0039*	2.45	2.45	16.56	0.0048
X22	1	12.40	12.40	13.13	0.0085*	9.19	9.19	17.98	0.0038	9.01	9.01	60.91	0.0001
X32	1	37.67	37.67	39.89	0.0004*	25.65	25.65	50.19	0.0002*	12.35	12.35	83.51	<0.0001*
Residual	7	6.61	0.94			3.58	0.51			1.04	0.15
Lack of fit	3	1.52	0.51	0.40	0.7630**	0.62	0.21	0.28	0.8397**	0.74	0.25	3.27	0.1414**
Pure Error	4	5.09	1.27			2.96	0.74			0.30	0.075
Cor Total	16	379.30				323.25				216.72

* means significant, ** means not significant.