TABLE 2.
Nucleotide sequences of PCR oligonucleotide primers and oligonucleotide probes for diarrheagenic E. coli strains
Category | Factor | PCR oligonucleotidesa | Reference | Oligonucleotide probe | Reference |
---|---|---|---|---|---|
ETEC | STI | TTAATAGCACCCGGTACAAGCAGG | 492 | GCTGTGAATTGTGTTGTAATCC | 457 |
CTTGACTCTTCAAAAGAGAAAATTAC | GCTGTGAACTTTGTTGTAATCC | ||||
LT | GGCGACAGATTATACCGTGC | 581 | GCGAGAGGAACACAAACCGG | 581 | |
CCGAATTCTGTTATATATGTC | |||||
EPEC | eae | —b | |||
EAF | CAGGGTAAAAGAAAGATGATAA | 214 | TATGGGGACCATGTATTATCA | 313 | |
TATGGGGACCATGTATTATCA | |||||
BFP | AATGGTGCTTGCGCTTGCTGC | 268 | GCTACGGTGTTAATATCTCTGGCG | 462 | |
GCCGCTTTATCCAACCTGGTA | |||||
EHEC | eae | CAGGTCGTCGTGTCTGCTAAA | 234 | ACTGAAAGCAAGCGGTGGTG | 691 |
TCAGCGTGGTTGGATCAACCT (O157:H7-specific) | |||||
SLTI | TTTACGATAGACTTCTCGAC | 223 | GATGATCTCAGTGGGCGTTC | 270 | |
CACATATAAATTATTTCGCTC (SLT-I AND II) | |||||
SLTII | As above | TCTGAAACTGCTCCTGTGTA | 270 | ||
Plasmid | ACGATGTGGTTTATTCTGGA | 223 | CCGTATCTTATAATAAGACGGATGTTGG | 223 | |
CTTCACGTCACCATACATAT | |||||
EIEC | ial | CTGGATGGTATGGTGAGG | 579 | CCATCTATTAGAATACCTGTG | 579 |
GGAGGCCAACAATTATTTCC | |||||
EAEC | Plasmid | CTGGCGAAAGACTGTATCAT | 576 | None | |
CAATGTATAGAAATCCGCTGTT |
Each primer is written 5′-3′. See the text for abbreviations and discussion.
No oligonucleotide primers have yet been described which will detect specifically all human EPEC strains. (See reference 234.)