TABLE 2.

Nucleotide sequences of PCR oligonucleotide primers and oligonucleotide probes for diarrheagenic E. coli strains

Category	Factor	PCR oligonucleotidesa	Reference	Oligonucleotide probe	Reference
ETEC	STI	TTAATAGCACCCGGTACAAGCAGG	492	GCTGTGAATTGTGTTGTAATCC	457
		CTTGACTCTTCAAAAGAGAAAATTAC		GCTGTGAACTTTGTTGTAATCC
	LT	GGCGACAGATTATACCGTGC	581	GCGAGAGGAACACAAACCGG	581
		CCGAATTCTGTTATATATGTC
EPEC	eae	—b
	EAF	CAGGGTAAAAGAAAGATGATAA	214	TATGGGGACCATGTATTATCA	313
		TATGGGGACCATGTATTATCA
	BFP	AATGGTGCTTGCGCTTGCTGC	268	GCTACGGTGTTAATATCTCTGGCG	462
		GCCGCTTTATCCAACCTGGTA
EHEC	eae	CAGGTCGTCGTGTCTGCTAAA	234	ACTGAAAGCAAGCGGTGGTG	691
		TCAGCGTGGTTGGATCAACCT (O157:H7-specific)
	SLTI	TTTACGATAGACTTCTCGAC	223	GATGATCTCAGTGGGCGTTC	270
		CACATATAAATTATTTCGCTC (SLT-I AND II)
	SLTII	As above		TCTGAAACTGCTCCTGTGTA	270
	Plasmid	ACGATGTGGTTTATTCTGGA	223	CCGTATCTTATAATAAGACGGATGTTGG	223
		CTTCACGTCACCATACATAT
EIEC	ial	CTGGATGGTATGGTGAGG	579	CCATCTATTAGAATACCTGTG	579
		GGAGGCCAACAATTATTTCC
EAEC	Plasmid	CTGGCGAAAGACTGTATCAT	576	None
		CAATGTATAGAAATCCGCTGTT

^aEach primer is written 5′-3′. See the text for abbreviations and discussion. 

^bNo oligonucleotide primers have yet been described which will detect specifically all human EPEC strains. (See reference 234.)