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Time course of IL-4 and IL-5 mRNA production. Cells were activated with PMA-A23187 and were incubated for various times before lysis for RNA extraction. IL-4 and IL-5 mRNAs were detected by RT-PCR, and band intensity was determined by laser densitometry. Each value represents the mean and SEM for four (IL-4) or three (IL-5) separate experiments.
