FIG. 6.

Effect of time of addition of dexamethasone. Cells were activated at time zero and 4 h later were harvested for RNA extraction. Dexamethasone was added at times ranging from 16 h prior to activation (−16) to immediately before cell harvest, i.e., 4 h after activation. RT-PCR was performed for IL-4 and IL-5. Quantitative data were determined as described in the legend to Fig. 3 and were expressed as a percentage of the values for the samples to which dexamethasone (dex) was added 4 h after activation. The data are the means and SEMs of two separate experiments.