| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ProLong gold antifade mountant with DAPI | Thermo Fisher Scientific | Cat# P36931 |
| CD31 (PECAM-1) monoclonal antibody (390), FITC, eBioscience (stock: 0.5 mg/mL; final: 5 μg/mL) | Thermo Fisher Scientific | Cat# 11-0311-85 |
| APC anti-mouse CD68 antibody (stock: 0.2 mg/mL; final 2 μg/mL) | BioLegend | Cat# 137008 |
| Anti-actin, α-smooth muscle-Cy3 antibody, mouse monoclonal (stock: 1.0–1.5 mg/mL; final 10–15 μg/mL) | MilliporeSigma | Cat# C6198 |
| Chemicals, peptides, and recombinant proteins | ||
| Buprenorphine, 0.05–0.1 mg/kg | Par Pharmaceutical | Cat# NDC42023-179-01 |
| PBS, phosphate-buffered saline, 10X solution, Fisher BioReagents | Fisher Scientific | Cat# BP3991 |
| Triton X-100 | Santa Cruz Biotechnology | CAS# 9002-93-1 |
| Calcium chloride solution (CaCl2) (stock: 1.0 M) | Honeywell Fluka | Cat# 21114-1L |
| Magnesium chloride (MgCl2) (stock: 25 mM) | Thermo Scientific | Cat# R0971 |
| Manganese(II) chloride (MnCl2), 50 g | Sigma-Aldrich | Cat# 244589-50G |
| Goat serum | MilliporeSigma | Cat# G9023-10ML |
| 0.9% sodium chloride for injection, USP | Hospira, Inc. | NDC 0409-4888-02 |
| Sterile water for injection, USP | Hospira, Inc. | NDC 0409-4887-23 |
| Invitrogen TRIzol LS reagent | Fisher Scientific | Cat# 10-296-010 |
| Paraformaldehyde solution, 4% in PBS, Thermo Scientific Chemicals | Thermo Fisher Scientific | Cat# J19943.K2 |
| Sucrose (EP/BP/NF), Fisher Chemical | Fisher Scientific | Cat# S2-500GM |
| O.C.T. compound | Fisher Scientific | Cat# 23-730-571 |
| Experimental models: Organisms/strains | ||
| Mus musculus: C57BL/6 mice (10- to 12-week-old males and females) | The Jackson Laboratory | Cat# 000664; RRID: IMSR_JAX:000664 |
| Mus musculus: Csf1rmercremer, FVB-Tg(Csf1rcre/Esr1∗)1Jwp/J (10- to 12-week-old males and females) | The Jackson Laboratory | Cat# 019098; RRID: IMSR_JAX:019098 |
| Mus musculus: IL-1 fl/fl (10- to 12-week-old males and females) | The Morrison Laboratory and the Brown University Mouse Transgenic and Gene Targeting Facility | Mantsounga et al.1 |
|
Mus musculus: Ai9 (RCL-tdT), B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J (10- to 12-week old males and females) |
The Jackson Laboratory | Cat# 007909; RRID: IMSR_JAX:007909 |
| Software and algorithms | ||
| moorLDI version 6.1 | Moor Instruments | https://www.moor.co.uk/en-us/products/control/moorldi2-research-software/ |
| ImageJ version: 2.0.0-rc-69/1.52p build:269a0ad53f date 2018-12-04T11:30:09 + 000 | Schneider et al., 2012 | https://imagej.net/software/fiji/downloads |
| Other | ||
| Instrument sterilization tray with silicone mat, 15 × 25.5 × 2 cm | World Precision Instruments | Cat# 500254 |
| Crosstex international pouch sterilization duo check, 12 × 18 in | Grayline Medical | Cat# SCL1218 |
| Dumont #5/45 forceps, standard/Dumoxel | Fine Science Tools | Cat# 11251-35 |
| Dumont forceps micro-blunted tips | Fine Science Tools | Cat# 11253-20 |
| Curved serrated forceps/Adson’s forceps from mouse surgical kit | Kent Scientific | Cat# INSMOUSEKIT |
| Vannas scissors, 8 cm, 5 mm blades, straight | World Precision Instruments | Cat# 14003 |
| Dissecting scissors, 10 cm, straight | World Precision Instruments | Cat# 14393 |
| Webster needle holder, serrated | World Precision Instruments | Cat# 14109 |
| AutoClip 9 mm wound closure clip | ALZET | Cat# 0009953 |
| Wound closure clamp | World Precision Instruments | Cat# 503292 |
| Covidien 9132 Curity all-purpose sponges, non-woven, 3-ply, 2″ × 2″ size | Vitality Medical | Cat# 9132 |
| Medium sheet, 40 × 71 in | Cardinal Health | Cat# 9355 |
| BWK7110, Boardwalk premium quality aluminum foil roll | Amazon | Cat# B00H615JA2 |
| Far infrared warming pad with controller | Kent Scientific | Cat# RT-0515 |
| Midmark VMR non-rebreathing anesthesia machine | Midmark | Cat# 91800218 |
| Sterile absorbent pad | HK Surgical | Cat# PD-X |
| 3M Transpore surgical tape, 1 in (width) | Fisher Scientific | Cat# 18-999-381 |
| 50 mL centrifuge tube, bag, sterile | Fisher Scientific | Cat# 50-202-003 |
| Nair hair remover lotion with soothing aloe & lanolin | Medline | Cat# C-H22329 |
| BD 1 mL TB syringe | Vitality Medical | Cat# 309626 |
| Povidone iodine prep solutions | Medline | Cat# MDS093906H |
| Decon CiDehol 70 isopropyl alcohol solution | Fisher Scientific | Cat# 04-355-71 |
| Puralube veterinarian ophthalmic ointment | Covetrus | Cat # 008897 |
| Sterile cotton swabs | Medline | Cat# MDS202095Z |
| Leica stereo microscope | Leica Biosystems | Model M80 |
| Sterile gloves | Thomas Scientific | Cat# 20A00C896, 20A00C 897, 20A00C898 |
| 7-0 DemeTECH DemeLENE polypropylene blue 18″ P-1 reverse cutting sutures | eSutures.com | Cat# PM197011F13M |
| High-resolution laser Doppler imager | Moor Instruments Ltd. | Cat# MOORLDI2-HIR |
| Heating pad | Harvard Apparatus | Cat# 55-7020 |
| Enterprise Technology Solutions latex-free mouse pad, 9 × 7.5, black | Fisher Scientific | Cat# 50-233-4833 |
| Heat lamp | Braintree Scientific | Cat# HL-1 120V |
| Kimberly-Clark Professional Kimtech Science Kimwipes delicate task wipers 1-ply | Fisher Scientific | Cat# 06-666 |
| Inotech Biosceince LLC IS-350 dry sterilizer | Fisher Scientific | Cat# NC9449759 |
| Flat razor blade | VWR | Cat# 55411-050 |
| Tissue-Tek Cryomold disposable vinyl specimen molds 25 × 20 × 5 mm | VWR | Cat# 25608-916 |
| Leica CM3050 S cryostat | Leica Biosystems | Model CM3050 S |
| Simport Scientific StainTray slide staining systems, black lid, only for M922 stain tray, M923-2 | Grayline Medical | Cat #M923-2 |
| ImmEdge pen | Vector Labs | Cat# H-4000 |
| Nikon ECLIPSE Ni-E microscope | Nikon | Model Ni-E |