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. 2025 May 5;6(2):103783. doi: 10.1016/j.xpro.2025.103783
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

ProLong gold antifade mountant with DAPI Thermo Fisher Scientific Cat# P36931
CD31 (PECAM-1) monoclonal antibody (390), FITC, eBioscience (stock: 0.5 mg/mL; final: 5 μg/mL) Thermo Fisher Scientific Cat# 11-0311-85
APC anti-mouse CD68 antibody (stock: 0.2 mg/mL; final 2 μg/mL) BioLegend Cat# 137008
Anti-actin, α-smooth muscle-Cy3 antibody, mouse monoclonal (stock: 1.0–1.5 mg/mL; final 10–15 μg/mL) MilliporeSigma Cat# C6198

Chemicals, peptides, and recombinant proteins

Buprenorphine, 0.05–0.1 mg/kg Par Pharmaceutical Cat# NDC42023-179-01
PBS, phosphate-buffered saline, 10X solution, Fisher BioReagents Fisher Scientific Cat# BP3991
Triton X-100 Santa Cruz Biotechnology CAS# 9002-93-1
Calcium chloride solution (CaCl2) (stock: 1.0 M) Honeywell Fluka Cat# 21114-1L
Magnesium chloride (MgCl2) (stock: 25 mM) Thermo Scientific Cat# R0971
Manganese(II) chloride (MnCl2), 50 g Sigma-Aldrich Cat# 244589-50G
Goat serum MilliporeSigma Cat# G9023-10ML
0.9% sodium chloride for injection, USP Hospira, Inc. NDC 0409-4888-02
Sterile water for injection, USP Hospira, Inc. NDC 0409-4887-23
Invitrogen TRIzol LS reagent Fisher Scientific Cat# 10-296-010
Paraformaldehyde solution, 4% in PBS, Thermo Scientific Chemicals Thermo Fisher Scientific Cat# J19943.K2
Sucrose (EP/BP/NF), Fisher Chemical Fisher Scientific Cat# S2-500GM
O.C.T. compound Fisher Scientific Cat# 23-730-571

Experimental models: Organisms/strains

Mus musculus: C57BL/6 mice (10- to 12-week-old males and females) The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664
Mus musculus: Csf1rmercremer, FVB-Tg(Csf1rcre/Esr1∗)1Jwp/J (10- to 12-week-old males and females) The Jackson Laboratory Cat# 019098; RRID: IMSR_JAX:019098
Mus musculus: IL-1 βfl/fl (10- to 12-week-old males and females) The Morrison Laboratory and the Brown University Mouse Transgenic and Gene Targeting Facility Mantsounga et al.1
Mus musculus: Ai9 (RCL-tdT), B6.Cg-Gt(ROSA)
26Sortm9(CAG-tdTomato)Hze/J (10- to 12-week old males and females)
The Jackson Laboratory Cat# 007909; RRID: IMSR_JAX:007909

Software and algorithms

moorLDI version 6.1 Moor Instruments https://www.moor.co.uk/en-us/products/control/moorldi2-research-software/
ImageJ version: 2.0.0-rc-69/1.52p build:269a0ad53f date 2018-12-04T11:30:09 + 000 Schneider et al., 2012 https://imagej.net/software/fiji/downloads

Other

Instrument sterilization tray with silicone mat, 15 × 25.5 × 2 cm World Precision Instruments Cat# 500254
Crosstex international pouch sterilization duo check, 12 × 18 in Grayline Medical Cat# SCL1218
Dumont #5/45 forceps, standard/Dumoxel Fine Science Tools Cat# 11251-35
Dumont forceps micro-blunted tips Fine Science Tools Cat# 11253-20
Curved serrated forceps/Adson’s forceps from mouse surgical kit Kent Scientific Cat# INSMOUSEKIT
Vannas scissors, 8 cm, 5 mm blades, straight World Precision Instruments Cat# 14003
Dissecting scissors, 10 cm, straight World Precision Instruments Cat# 14393
Webster needle holder, serrated World Precision Instruments Cat# 14109
AutoClip 9 mm wound closure clip ALZET Cat# 0009953
Wound closure clamp World Precision Instruments Cat# 503292
Covidien 9132 Curity all-purpose sponges, non-woven, 3-ply, 2″ × 2″ size Vitality Medical Cat# 9132
Medium sheet, 40 × 71 in Cardinal Health Cat# 9355
BWK7110, Boardwalk premium quality aluminum foil roll Amazon Cat# B00H615JA2
Far infrared warming pad with controller Kent Scientific Cat# RT-0515
Midmark VMR non-rebreathing anesthesia machine Midmark Cat# 91800218
Sterile absorbent pad HK Surgical Cat# PD-X
3M Transpore surgical tape, 1 in (width) Fisher Scientific Cat# 18-999-381
50 mL centrifuge tube, bag, sterile Fisher Scientific Cat# 50-202-003
Nair hair remover lotion with soothing aloe & lanolin Medline Cat# C-H22329
BD 1 mL TB syringe Vitality Medical Cat# 309626
Povidone iodine prep solutions Medline Cat# MDS093906H
Decon CiDehol 70 isopropyl alcohol solution Fisher Scientific Cat# 04-355-71
Puralube veterinarian ophthalmic ointment Covetrus Cat # 008897
Sterile cotton swabs Medline Cat# MDS202095Z
Leica stereo microscope Leica Biosystems Model M80
Sterile gloves Thomas Scientific Cat# 20A00C896, 20A00C
897, 20A00C898
7-0 DemeTECH DemeLENE polypropylene blue 18″ P-1 reverse cutting sutures eSutures.com Cat#
PM197011F13M
High-resolution laser Doppler imager Moor Instruments Ltd. Cat# MOORLDI2-HIR
Heating pad Harvard Apparatus Cat# 55-7020
Enterprise Technology Solutions latex-free mouse pad, 9 × 7.5, black Fisher Scientific Cat# 50-233-4833
Heat lamp Braintree Scientific Cat# HL-1 120V
Kimberly-Clark Professional Kimtech Science Kimwipes delicate task wipers 1-ply Fisher Scientific Cat# 06-666
Inotech Biosceince LLC IS-350 dry sterilizer Fisher Scientific Cat# NC9449759
Flat razor blade VWR Cat# 55411-050
Tissue-Tek Cryomold disposable vinyl specimen molds 25 × 20 × 5 mm VWR Cat# 25608-916
Leica CM3050 S cryostat Leica Biosystems Model CM3050 S
Simport Scientific StainTray slide staining systems, black lid, only for M922 stain tray, M923-2 Grayline Medical Cat #M923-2
ImmEdge pen Vector Labs Cat# H-4000
Nikon ECLIPSE Ni-E microscope Nikon Model Ni-E