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. 1998 Jan;5(1):50–57. doi: 10.1128/cdli.5.1.50-57.1998

FIG. 2.

FIG. 2

Mapping of MAb RA-E7 reactivity with MBPMSG truncated fusion proteins by SDS-PAGE and immunoblotting. (A) Coomassie blue-stained gel. (B) Immunoblot with anti-MBP polyclonal antisera. (C) Immunoblot with RB-E7. Lanes 1, MBP; lanes 2, native MSG; lanes 3, MBPMSG41–561; lanes 4, MBPMSG41–373; lanes 5, MBPMSG41–324; lanes 6, MBPMSG41–290; lanes 7, MBPMSG41–278; lanes 8, MBPMSG193–322. (D) ELISA analysis of MAb RA-E7 reactivity with MBPMSG-B truncated fusion proteins. Wells of 96-well microtiter plates were coated with the fusion proteins or MBP alone. The numbers correspond to the lanes described above. (E) Schematic of mapping of the epitope reactive with MAb RA-E7. The numbers in parentheses correspond to the lanes described above.