TABLE 2.
FACS analysis of phagocytosis of C. neoformans by PMNs cocultured with IgM antibodies and rabbit and human complement sourcesa
| Serum and/or antibody | Phagocytosis (% FITC-labeled PMNs) |
|---|---|
| None | 3.41 ± 1.02 |
| 10% heated rabbit serum | 5.98 ± 2.28 |
| 10% unheated rabbit serum | 10.52 ± 2.66 |
| Myeloma IgM | 5.03 ± 1.89 |
| Myeloma IgM + 10% unheated rabbit serum | 10.52 ± 2.78 |
| Myeloma IgM + 10% heated rabbit serum | 7.04 ± 1.76 |
| 2E9 | 4.52 ± 2.48 |
| 2E9 + 10% unheated rabbit serum | 37.04 ± 9.76b |
| 2E9 + 10% heated rabbit serum | 5.71 ± 3.99 |
| 10% factor-B-deficient serum | 12.85 ± 4.34 |
| 2E9 + 10% factor-B-deficient serum | 57.37 ± 21.61c |
| RC2 + 10% factor-B-deficient serum | 11.26 ± 5.23 |
a
The E/T ratio for these experiments was 1:10 (PMNs to yeast cells). Results for rabbit serum represent five different experiments, and those for factor-B-deficient serum represent three different experiments. P values were determined by the Mann-Whitney U test.
b
P < 0.01 versus heated rabbit serum.
c
P < 0.005 versus factor-B-deficient serum.