FIG. 7.

Stat5-GR association in in vitro-cultured cells and organs. A 500-μg amount of whole-cell extracts made from HC11 cells, lactating mammary gland (MG), liver, and NIH 3T3 cells was immunoprecipitated (ip) with Stat5a antiserum (A) or Stat5b antiserum (B) and resolved by SDS-10% PAGE. The membrane was incubated sequentially with GR and Stat5a (A) or Stat5b (B) antiserum. (C) An 80-μg amount of the protein extracts described above (lanes 1 to 4) or 500 μg of the same extracts immunoprecipitated with GR antiserum (lanes 5 to 8) was resolved by SDS-10% PAGE and probed for Stat3 with specific antiserum. Arrows indicate the proteins shown on the Western Western blot (WB). Ig, immunoglobulin.