TABLE 2.
Allele specificity of the MRP21 suppressor mutations
| Relevant genotype | Growth with MRP21 allelea:
|
||
|---|---|---|---|
| MRP21 | MRP21-1 | MRP21-3 | |
| COX3, COX2 | ++++ | ++++ | ++++ |
| cox3-15 | ± | +++ | +++ |
| pet54-A244, cox3-15 | − | + | ND |
| cox3-438 | − | − | − |
| cox3-1 | + | ± | ++ |
| cox2-11 | ± | ++ | ++ |
| cox2-105 | ± | + | ++ |
| cox2-12 | − | − | − |
| cox2-10 | ± | ± | + |
The number of + signs indicates relative level of respiratory growth, determined by measuring the colony size on YPEG medium at 30°C, except for cox3-15 mutants, which were incubated at 13.5°C. −, no growth; ND, not determined. Suppression was tested in haploids, except with cox2-12, cox2-105, and cox3-438, for which dominant suppression in heterozygous diploids is reported. References for the alleles listed in the table are as follows: cox3-15 (12); pet54-A244 (6); cox3-438 (67); cox3-1 (26); cox2-11 (45); cox2-105 (22); cox2-12 (45); cox2-10 (47). The following alleles showed no genetic interaction (neither suppression nor synthetic defects) with MRP21-1: cox3-13 and -14 (67); cox3-209 (13); cox2-13 (45); cox2-103, cox2-106, cox2-107, and cox2-108 (22); MRP51-1, MRP51-2, MRP51-3, MRP51-4, and MRP51-5 (this study); pet54-A244 (6); PET122-L195 (12); PET122-I175 and PET122-V211 (6); oli1-h45 (51).