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Plasmid-based synthesis of EcoRI and HO causes cell killing in rad9 mutants but not in Rad+ or rad52 cells. Cells grown in Gal + Glu − Ura medium (maintaining selection for the plasmid) were rescued onto synthetic Glu-Com plates as described in Materials and Methods. Symbols: □, pRS316; ◊, pGALHO; ○, YCpGal:Rlb.
