TABLE 2.
Plating efficiencies of cells expressing EcoRI or HOa
| Strain | PE on Gal/ PE on Glu | Fold decrease (rad52 vs RAD52) | Refer- ence |
|---|---|---|---|
| RAD52 (pRS316) | 1.06 | ||
| rad52 (pRS316) | 0.89 | 1.2 | |
| RAD52 (YCpGal:Rlb) | 0.32 | ||
| rad52 (YCpGal:Rlb) | 5.1 × 10−4 | 627.5 | |
| RAD52 (Δlys2::GALEcoRI) | 0.71 | ||
| rad52 (Δlys2::GALEcoRI) | 18 × 10−4 | 394.4 | |
| RAD52 (Δhis3::GALEcoRI) | 0.52 | ||
| rad52 (Δhis3::GALEcoRI) | 14 × 10−4 | 371.4 | |
| RAD52 (pGALHO) | 0.46 | ||
| rad52 (pGALHO) | 3.3 × 10−4 | 1,394 | |
| Previous resultsb | |||
| rad52 (pGALHO) | ∼1 × 10−4 | 14 | |
| rad52 (pGALHO) | (1–4) × 10−4 | 31 | |
| rad52 (pGALHO) | (0.1–1) × 10−4 | 28c | |
| rad52 (pGALHO) | 16 × 10−4 | 45 |
a
Plating efficiencies (PE) (plating CFU/hemacytometer counts) were determined at 30°C with synthetic Glu − Ura and Gal + Glu − Ura plates for plasmid-containing strains and YPD and YPD+2%Gal plates for cells containing integrated fusions. T334 (reg1-501) and derivatives used in the assay are described in the text.
b
Previously published plating efficiencies for REG1 rad52 strains obtained by similar methods.
c
Gal/Glu plating efficiencies were determined at 25 and 33°C.