TABLE 3.
Stabilities of HO and EcoRI expression plasmidsa
| Vector | Strain | % of plasmid-containing
cells at time (h):
|
||
|---|---|---|---|---|
| 0 | 12 | 24 | ||
| pRS316 | Rad+ | 67.5 | 60.5 | 88.9 |
| Δrad52 | 47.2 | 66.7 | 67.8 | |
| Δrad9 | 52.9 | 45.7 | 81.0 | |
| pGALHO | Rad+ | 55.4 | 48.2 | 52.1 |
| Δrad52 | 45.7 | 27.0 | 3.8 | |
| Δrad9 | 61.9 | 60.2 | 51.2 | |
| YCpGal:RIb | Rad+ | 72.9 | 45.7 | 18.1 |
| Δrad52 | 55.5 | 41.5 | 7.6 | |
| Δrad9 | 75.0 | 45.3 | 15.8 | |
a
The mean percentage of cells retaining the plasmid is shown. Aliquots of cells grown in Gal + Glu − Ura liquid medium were removed and spread directly to synthetic Glu-Com and Glu − Ura plates.