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Analysis of c-myc mRNA expression. Control (+/+) and Stat6-deficient (−/−) lymphocytes were activated with ConA for 72 h and then incubated for one additional hour in the presence (+) or absence (−) of IL-4. Ten micrograms of total RNA was loaded in each lane. Following hybridization with a c-myc probe, the blot was stripped and rehybridized with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe as a control for RNA loading.
