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p27Kip1 expression in control and Stat4-deficient lymphocytes. Control (+/+) and Stat4-deficient (−/−) lymphocytes were activated with anti-CD3 for 72 h and incubated for an additional 30 h in the presence or absence (−) of the indicated cytokine. Cell extracts were analyzed by immunoblotting as described in the legend for Fig. 5.
