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Gel filtration chromatography of Hop and Hop-Hsc70 complex. Hop (60 μg) was incubated alone (Hop) or in the presence of Hsc70 (73 μg) (Hop/Hsc70) for 1 h at 4°C, followed by gel filtration chromatography on a Superose 12 column. Amounts of Hop were quantified by densitometry of Coomassie blue-stained SDS-polyacrylamide gels.
