FIG. 4.

DNA sequence analysis of cloned broken coding- and signal-end LM-PCR fragments. Broken coding-end fragments were excised from agarose gels, reamplified, purified, and cloned into the vector pBSK. Randomly selected coding-end clones were sequenced. The arrows above the germ line sequences indicate sites of linker ligation determined by sequencing. The numbers above the arrows indicate the frequencies of individual sequences from sets of consecutive sequences. The solid vertical bar indicates the RSS–coding-segment junction, with arrows to the right indicating coding ends and those to the left indicating signal ends. The RSS heptamer and nonamer sequences are boxed. Signal-end sequences were reported previously and are shown for comparison only (28).