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Recipe for dissection solution according to Zhang et al., 20138
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 103 mM | 3.010 g |
| KCl | 3 mM | 0.112 g |
| MgCl2 | 4 mM | 0.407 g |
| TES | 5 mM | 0.573 g |
| Sucrose | 7 mM | 1.198 g |
| Glucose | 10 mM | 0.901 g |
| Trehalose | 10 mM | 1.892 g |
| NaHCO3 | 26 mM | 1.092 g |
| NaH2PO4 | 1 mM | 0.069 g |
| Milli-Q water | – | fill up to 500 ml |
Adjust pH to 7.25 with 1 M HCl.
