FIG. 4.

Nrl is a candidate for binding the ζ lens promoter MARE. (a) Antiserum to Nrl reduces the formation of the UB-A complex in mouse lens nuclear extract. Lanes show EMSA with the −229 to −188 probe with no added antiserum, with antiserum to Nrl, and with nonimmune serum. Other antisera were also tested and had no effect on complex formation. (b) Cotransfection with the Nrl expression vector pMTNRL increases ζ lens promoter activity in N/N1003A lens cells. The relative CAT reporter activity for the −229/+70.CAT construct cotransfected either with the empty pMT3 plasmid (−229) or with the Nrl expression plasmid pMTNRL (−229 + Nrl) is shown.