FIG. 4.

Repression of basal as well as RelA (p65)-stimulated NF-κB2 promoter activity by RBP-2N depends on a functional RBP-Jκ consensus sequence. (A) Portions (2 μg) of reporter constructs pASwt (black bars) and pAS-SL49 (open bars) were cotransfected into COS-7 cells with increasing amounts of an RBP-2N expression plasmid. (B and C) Portions (2 μg) of reporter constructs pASwt (black bars) and pAS-SL49 (open bars) were cotransfected with 500 ng of pRSVRelA and increasing amounts of CMV-RBP-2N into COS-7 cells (B) or Jurkat-T cells (C). Luciferase activity was determined from 100 μg of total cell extract, and the basal promoter activity of each construct was set to one. Mean values and standard deviations for at least four independent experiments are shown.