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. 2025 Jun 6;14:RP104074. doi: 10.7554/eLife.104074

Figure 7. Knockout of the bcd uORF reduces offspring number and starvation resistance.

Figure 7.

(A) Comparison of the hatching rates (%) of mutant and WT offspring (n=20, Wilcoxon rank-sum test; ***, p<0.001). (B) The offspring number per maternal parent in different crosses over 10 days at 25 °C. Asterisks indicate significant differences between various crosses and crosses of WT females with WT males (n=20, Wilcoxon rank-sum test; *, p<0.05; **, p<0.01; ***, p<0.001; n.s., p>0.05). The different crosses were denoted as the x-axis labels. (C) The offspring number per maternal parent in different crosses over 10 days at 29 °C. (D) Survival curves of WT and mutant adult flies of females (left) and males (right) under starvation conditions. The black line represents the WT, the red line represents the uKO1/uKO1 mutant, and the blue line represents the uKO2/uKO2 mutant. Asterisks indicate significant differences compared to the WT. (n=200, log-rank test; ***, p<0.001; n.s., p>0.05).

Figure 7—source data 1. Raw numerical data underlying the figures.