FIG. 1.

Sequence analysis of TFC6 and characterization of anti-τ91 antibodies. (A) Deduced amino acid sequence of TFC6. The four microsequenced peptides used for database searching are underlined (GenBank no. U28372). The TFC6 mutation, E330K, responsible for tfc3-G349E suppression is boxed. (B) Amino acid analysis of τ91. The acidic-basic map shows the position of acidic (A) (D, short bars; E, long bars), and basic (B) (R, long bars; K, short bars; H, smallest bars) amino acids. In the Cys-His map, histidine and cysteine residues are plotted as long and short bars, respectively. (C) Characterization of anti-τ91 antibodies by Western blotting. Lane 1, the polypeptide content of a tDNA affinity-purified TFIIIC fraction was analyzed in a silver-stained SDS–8% polyacrylamide gel. (Lanes 2 and 3, ∼100 ng of TFIIIC (lane 2) and ∼20 ng of purified rTFC6p (rτ91) (lane 3) were subjected to SDS-PAGE, electroblotted to a nitrocellulose membrane, and probed with anti-τ91 antibodies. Immune complexes were visualized with an ECL kit (Amersham). The positions of 91- and 75-kDa proteins are indicated. The amino acid sequence, acidic-basic map, and Cys-His map are printouts from DNA Strider 1.2 software (37).