FIG. 1.

Induction of C/EBPδ and C/EBPβ mRNAs by IL-6 or IFN-γ in hepatoma cell lines. (A) Northern blot analysis of 20 μg of total RNA from Hep3B or HepG2 cells cells treated with IL-6 or IFN-γ for the indicated times. Duplicate blots were hybridized with C/EBPδ or C/EBPβ probes and then with cyclophilin. (B) Northern blotting analysis of 20 μg of total RNAs from control Hep3B cells (lane 1), cells treated with cycloheximide (CHX) for 30, 60, and 120 min (lanes 2 to 4), and cells pretreated with cycloheximide for 30, 60, and 120 min followed by the addition of IL-6 for 2 h (lanes 5 to 7), IL-6 alone for 2 h (lane 8), or cycloheximide and IL-6 concurrently for 2 h (lane 9). The blots were hybridized sequentially with the indicated probes.