Table 2.

Effects of folic acid supplementation on cardiac development in offspring of various animal models.

Animal	Teratogen	Folic aicd dose	Outcome	Literature
Mice	Retinoic acid competitive antagonist	4 mg/kg(i.p)	+	Amati et al. (2010)
Mice	Retinoic acid competitive antagonist	4 mg/kg(i.p)	+	Cipollone et al. (2009)
Mice	Alcohol	10.5 mg/kg (high dose) or 6.2 mg/kg (medium dose) FA supplementation diet	+	Serrano et al. (2010)
Mice	Li	10.5 mg/kg FA supplemented diet	+	Han et al. (2009)
Mice	Li	10.5 mg/kg FA supplemented diet	+	Han et al. (2016)
Mice	HCY	10.5 mg/kg FA supplemented diet	+	Serrano et al. (2010)
Mice	HCY	10.5 mg/kg FA supplemented diet	+	Han et al. (2016)
Mice	-	40 mg/kg FA supplementation diet	-	Pickell et al. (2011)
Mice	-	20 mg/kg FA supplementation diet	-	Mikael et al. (2013)
Rat	Arsenic	0.53 mg/kg,5.3 mg/kg,10.6 mg/kg of FA supplementation diet	+	Lin et al. (2018)
Rat	Arsenic	0.53 mg/kg,5.3 mg/kg,10.6 mg/kg of FA supplementation diet	+	Na et al. (2020)
Quail	Alcohol	3.2 μg each egg	+	Ford et al. (2021)
Quail	Alcohol	10 μg/mL added to the medium	+	Serrano et al. (2010)
Chick	Li	10 μg/mL added to the medium	+	Han et al. (2009)
Chick	HCY	10 μg/mL added to the medium	+	Han et al. (2009)
Zebrafish	PM2.5 organic extract	0.02、0.05、0.2 μM	+/−	Yue et al. (2017)
Zebrafish	Selenite	0.01、0.1、1 mM	+	Ma et al. (2012)
Zebrafish	-	1、2、3、4、5 mM	-	Han et al. (2021)
Zebrafish	-	0.25 mM, 0.5 mM, 1 mM, 2.5 mM, 5 mM	-	Lian et al. (2022)

FA: folic acid; Bolded indicates the presence of negative effects and their doses; +No. means reducing CHD risk, while -No. means increasing CHD risk.