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One-hybrid analysis to localize the activation domain of Mei4. (Left panel) Construction of fusion proteins between a Gal4 DNA-binding domain and Mei4 proteins. Numerals represent the positions of amino acid residues from the N terminus. S. cerevisiae SFY526 was transformed with the indicated plasmids. fkh, forkhead. (Right panel) β-Galactosidase activities, with standard deviations, for three independent transformants.
