TABLE 2.
Mating and sporulation of mei4-defective and mei4-overexpressing strains
| Expta | Strain | Relevant genotype | Zygotes (%) | Asci (%)b
|
Nuclear type of zygotes (%)c
|
||||
|---|---|---|---|---|---|---|---|---|---|
| 4 spore | 2 spore | 1N(r) | 1N(ht) | 2N | 3-4N | ||||
| 1 | JZ878 | h90 mei4+ | 10 | 60 | 0 | 4 | 5 | 1 | 90 |
| YW917 | h90 mei4Δ | 65 | 0 | 0 | 76 | 24 | 0 | 0 | |
| YW917 | h90 mei4Δ [pREP(mei4+)] | 13 | 47 | 8 | 13 | 5 | 2 | 80 | |
| 2 | YW917 | h90 mei4Δ [pAL19] | 69 | 0 | 0 | 28 | 71 | 1 | 0 |
| YW917 | h90 mei4Δ [pAL(mei4+)] | 25 | 29 | 5 | 16 | 32 | 20 | 32 | |
| YW917 | h90 mei4Δ [pAL(mei4-K85Q)] | 56 | 0 | 0 | 38 | 61 | 1 | 0 | |
| YW917 | h90 mei4Δ [pAL(mei4-W115S)] | 63 | 0 | 0 | 40 | 60 | 0 | 0 | |
| YW917 | h90 mei4Δ [pAL(mei4-F125D)] | 55 | 0 | 0 | 37 | 62 | 1 | 0 | |
a
In experiment 1 cells were cultured on SSA medium for 2 days; in experiment 2 cells were incubated in PM liquid medium lacking N for 12 h.
b
Two- and four-spore asci were differentially counted.
c
1N(r), mononucleate zygotes containing a round nucleus; 1N(ht), mononucleate zygotes with a horsetail nucleus; 2N, binucleate zygotes; 3-4N, tri- or tetranucleate zygotes.