TABLE 2.

Effects of different types of proteasome inhibitors on the cellular content of trehalose^a

Inhibitor	Trehalose (nmol/μg of protein)
None (0.1% DMSO)	1.76 ± 0.38
MG132 (50 μM)	4.62 ± 1.74
β-Lactone (20 μM)	3.46 ± 1.18

^aExponentially growing ise1 cells at 30°C were treated with MG132 (50 μM), β-lactone (20 μM), or 0.1% DMSO (control) for 2 h, and then cellular trehalose contents were measured as described in Materials and Methods. Data presented are mean values ± SD from three independent experiments.