FIG. 1.

Construction and purification of Pab1p variants. (A) Schematic diagram of the Pab1p deletion constructs used in this study. The following amino acids were omitted from each of the constructs: Pab1-ΔRRM1p, 15 to 129; Pab1-ΔRRM2p, 132 to 216; Pab1-ΔRRM3p, 218 to 311; Pab1-ΔRRM4p, 316 to 427; Pab1-ΔCtermp, 430 to 584; and Pab1-105p, 218 to 584. WT, wild type. (B) Purified Pab1p variants. An SDS–10% polyacrylamide gel stained with Coomassie brilliant blue is shown. Positions of molecular weight standards are indicated to the right.