FIG. 3.

RRM2 is required for Pab1p binding to eIF4G. Glutathione resins containing the Pab1p-binding region of eIF4G1 (GST-eIF4G1/187-300p) (A) or eIF4G2 (GST-eIF4G2/200-315p) (B) were incubated with poly(A) and the indicated Pab1p variant. Eluates from these resins were then resolved by SDS-PAGE (12% gel) and visualized by Coomassie brilliant blue staining. Initial concentrations for the Pab1p variants in the binding reaction were 1.5 μM except for Pab1-ΔRRM1p and Pab1-105p, which were at 3 μM. Poly(A) was used at a concentration of 58 μM AMP. WT, wild type.