Abstract
Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.
Full Text
The Full Text of this article is available as a PDF (2.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashworth J. M., Sussman M. The appearance and disappearance of uridine diphosphate glucose pyrophosphorylase activity during differntiation of the cellular slime mold Dictyostelium discoideum. J Biol Chem. 1967 Apr 25;242(8):1696–1700. [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douglas H. C., Hawthorne D. C. Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast. Genetics. 1966 Sep;54(3):911–916. doi: 10.1093/genetics/54.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Free S. J., Schimke R. T. The structural gene for alpha-mannosidase-1 in Dictyostellium discoideum. Genetics. 1976 Oct;84(2):159–174. doi: 10.1093/genetics/84.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freeze H., Loomis W. F. Isolation and characterization of a component of the surface sheath of Dictyostelium discoideum. J Biol Chem. 1977 Feb 10;252(3):820–824. [PubMed] [Google Scholar]
- Harrington B. J., Raper K. B. Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds. Appl Microbiol. 1968 Jan;16(1):106–113. doi: 10.1128/am.16.1.106-113.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]