FIG. 1.

Effects of Alu RNAs on a transiently coexpressed reporter gene. (A) 293 cells were transiently cotransfected with a luciferase reporter gene (0.1 μg) and the indicated quantity of a clone overexpressing 7SL, scAlu-like, flAlu (clone XAT), or VAI RNA or plasmid pUC for a 293 cell control and with additional pUC DNA to provide a total of 40 μg of DNA for each sample. Luciferase activities were assayed at 24 h after transfection and are reported in arbitrary luminosity units. (B) The amounts of luciferase mRNA were assayed by primer extension in cells transfected with 0.1, 2.5, and 10 μg of clones overexpressing the indicated RNA.