FIG. 2.
Effect of rgd1Δ mutation on expression of the reporter gene PST1-lacZ during growth and after low-pH shock. The wild-type (square) and rgd1Δ (triangle) strains in the X2180 background were transformed with YEp357R-PST1. (A) Expression of PST1-lacZ (open symbols) in both strains was determined by measuring β-galactosidase during growth (closed symbols) in minimal medium. (B) Cells were grown in minimal medium up to an OD600 of 0.5, and the pH was brought from 3.5 to 2.8. PST1-lacZ expression was measured for 6 hours after the acid shock.