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Conservation and abundance of histone H3 K36 methylation. We resolved 1 μg of Xenopus laevis recombinant histone H3 and 5 μg of total core histones from the species indicated on 15% SDS-PAGE gels, transferred to a polyvinylidene difluoride membrane, and probed with anti-Me2K36 and anti-Me2K4. Identical samples were examined in parallel by Coomassie staining to show histone loading. The asterisk indicates H3 breakdown products that are typically observed.
