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. 2005 Aug;4(8):1446–1454. doi: 10.1128/EC.4.8.1446-1454.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 6. — SpSet2 rescues K36 methylation in S. cerevisiae. Whole-cell extracts (WCEs) were prepared from S. cerevisiae wild-type and set2Δ strains that were transformed with an empty vector or the indicated Set2-Flag constructs. These extracts were immunoprecipitated with anti-Flag antibody followed by immunoblot analysis using antibodies directed against unmodified CTD (8WG16, anti-unmod CTD), Ser5-phosphorylated CTD (H14, anti-Ser5P), or Flag (anti-Flag). The locations of Pol II and Set2-Flag proteins are indicated. All input extracts showed equivalent levels of Pol II (data not shown). Nuclear extracts prepared from the same strains were immunoblotted with an antibody directed against K36 dimethylation or an antibody specific for K4 dimethylation (used as a loading control). The asterisk indicates H3 breakdown products that were observed.