FIG. 5.

hH3^K36L allele confers phenotypes similar to the set-2^RIP1 mutation. (A) Sequence of histone H3 amino-terminal tail. Lysines that are methylated are indicated in bold. (B) Strategy used for replacement of wild-type hH3 with hH3^K36L. (C) Southern analysis to confirm replacement. DNA isolated from wild-type hH3 (N2836) and hH3^K36L (N2970) was digested with HindIII. The blot was probed with a fragment indicated in B. The single ∼5.1-kb band indicates that the wild-type hH3 gene in N2836 was replaced by the mutated hH3^K36L::inl⁺ allele. (D and E) Poor growth and conidiation of the hH3^K36L strain. Wild-type (N150) and hH3^K36L (N2970) strains were grown as described for Fig. 2. Linear growth of the wild type (N150: square) and two hH3^K36L transformants (N2970 [triangles] and N2975 [circles]) were measured at 32°C in growth tubes.