TABLE 5.
Phenotypic regulation of gene expression assessed by Northern blot analysis reflects promoter activity assessed in cells transformed with promoter-luciferase fusions
| Gene | Northern blotting result | RLUC sp act (104 RLUC units/30 s/μg protein)a
|
RLUC result | Fold difference of RLUC comparison
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Strain 40F1 (a)
|
Strain 12F1 (α)
|
||||||||
| Wh | DB | Wh | DB | Comparison | MTLa | MTLα | |||
| PMP3 | DB > Wh | 2.8 ± 1.6 | 21.1 ± 7.9 | 1.1 ± 0.5 | 4.8 ± 1.8 | DB > Wh | DB/Wh | 7.5 | 4.3 |
| TAR1 | DB > Wh | 1.6 ± 0.8 | 18.6 ± 8.2 | 1.3 ± 0.8 | 12.4 ± 5.9 | DB > Wh | DB/Wh | 11.6 | 9.5 |
| ECM17 | DB > Wh | 1.2 ± 0.6 | 6.9 ± 2.6 | 1.2 ± 0.6 | 6.5 ± 2.8 | DB > Wh | DB/Wh | 5.7 | 6.1 |
| HSP104 | DB > Wh | 1.9 ± 0.9 | 17.0 ± 8.4 | 1.2 ± 0.7 | 7.3 ± 4.6 | DB > Wh | DB/Wh | 8.9 | 6.1 |
| APC9 | Wh > DB | 13.1 ± 5.1 | 2.2 ± 1.1 | 14.0 ± 6.3 | 2.3 ± 1.5 | Wh > DB | Wh/DB | 5.9 | 6.1 |
| SUT1 | Wh ≅ DB | 4.3 ± 2.4 | 3.5 ± 2.0 | 2.6 ± 1.7 | 2.9 ± 1.8 | Wh ≅ DB | Wh/DB | 1.2 | 0.9 |
| TEF4 | Wh ≅ DB | 1.9 ± 1.1 | 1.5 ± 0.8 | 1.5 ± 0.5 | 1.9 ± 0.9 | Wh ≅ DB | Wh/DB | 1.3 | 0.8 |
a
The basal RLUC activity of strains harboring the promoterless vector pH12.7 targeted to the HO gene ranged between 5.9 × 103 and 6.2 × 103 units. This was subtracted from all RLUC activities obtained with promoter-containing constructs. Each measurement shown is the mean (± standard deviation) of six measurements of relative luminescence, three from each of two independent transformants.