FIG. 4.

Hypha formation assays of biofilm-defective mutants. Each strain was incubated in M199 medium, pH 8, at 37°C for 12 h. Culture samples were visualized at a ×400 magnification to visualize yeast cells and hyphae. All images are presented at the same magnification. Strains include the His⁺ reference strain DAY185 (A), an suv3/suv3 insertion mutant (B) and its SUV3 reconstituted derivative (C), a nup85/nup85 insertion mutant (D) and its NUP85 reconstituted derivative (E), an mds3/mds3 insertion mutant (F) and its MDS3 reconstituted derivative (G), and a kem1/kem1 insertion mutant (H) and its KEM1 reconstituted derivative (J). All of the insertion mutants were rendered His⁺ as described in the Fig. 2 legend.