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. 2005 Aug;4(8):1396–1402. doi: 10.1128/EC.4.8.1396-1402.2005

FIG. 2.

FIG. 2.

Function of Mcs4 in hydrogen peroxide versus arsenite stress. (A) Six-histidine-tagged Spc1::HA was purified from wild-type or mcs4(D412N) cells treated with 0.5 mM sodium arsenite or 1 mM hydrogen peroxide for 5 to 15 min. Phosphorylated Spc1 (Spc1-P) was detected using anti-phosphorylated p38. Spc1::HA was used as a loading control. (B) Phosphorylated Spc1 was detected from extracts obtained from wild-type or mcs4(D412N) cells treated with 0.5 mM sodium arsenite or 1 mM hydrogen peroxide for up to 60 min. Spc1::HA was used as a loading control.