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Pyp1 participates in arsenite signaling. Spc1 phosphorylation (Spc1-P) was monitored in the wild type and in wis4Δ win1-1, wis4Δ win1-1 pyp1Δ, and wis4Δ win1-1 pyp2Δ mutants. Cells were treated with 0.5 mM sodium arsenite. Spc1::HA was used as a loading control.
