TABLE 1.
C. albicans strains used in this study
| Strain | Parent | Relevant genotype or characteristicsa | Reference or source |
|---|---|---|---|
| SC5314 | Wild-type strain | 12 | |
| CAI4 | SC5314 | ura3Δ::imm434/ura3Δ::imm434 | 10 |
| TETG25A and -B | CAI4 | ADH1/adh1::Ptet-caGFPb | This study |
| TETC36A and -B | CAI4 | ADH1/adh1::Ptet-CDC42D118Ab | This study |
| TETN42A and -B | CAI4 | ADH1/adh1::Ptet-NRG1b | This study |
| TETMa46A and -B | CAI4 | ADH1/adh1::Ptet-MTLa2b | This study |
| WO-1 | Wild-type strain, MTLα | 37 | |
| WUM5A | WO-1 | ura3Δ::FRT/ura3Δ::FRT | 42 |
| WTETG40A and -B | WUM5A | OP4/op4::Ptet-caGFPb | This study |
| WTETMa41A and -B | WUM5A | OP4/op4::Ptet-MTLa1b | This study |
| WTETMa43A and -B | WUM5A | OP4/op4::Ptet-MTLa2b | This study |
| MTLa/Δmtlα | CAI4 | MTLa/mtlαΔ | 17 |
| ΔmtlαTETG40A and -B | MTLa/Δmtlα | OP4/op4::Ptet-caGFPb | This study |
| ΔmtlαTETMa43A and -B | MTLa/Δmtlα | OP4/op4::Ptet-MTLa2b | This study |
| SMC4 | CAI4 | CDC42/cdc42Δ::FRT ACT1/act1::FRT-CDC42-MPAR-FRT | 25 |
| SMC4TF23A and -B | SMC4 | ADH1/adh1::Ptet-ecaFLPb | This study |
| SMC6 | CAI4 | cdc42Δ::FRT/cdc42Δ::FRT ACT1/actl::FRT-CDC42-MPAR-FRT | 25 |
| SMC6TF23A and -B | SMC6 | ADH1/adh1::Ptet-ecaFLPb | This study |
| F2 | Clinical isolate, fluconazole sensitive | 11 | |
| F2NIM1A and -B | F2 | ADH1/adh1::Ptet-caGFPb | This study |
| F5 | Clinical isolate, fluconazole resistant, MDR1 overexpression | 11 | |
| F5NIM1A and -B | F5 | ADH1/adh1::Ptet-caGFPb | This study |
| G2 | Clinical isolate, fluconazole sensitive | 11 | |
| G2NIM1A and -B | G2 | ADH1/adh1::Ptet-caGFPb | This study |
| G5 | Clinical isolate, fluconazole resistant, MDR1 overexpression | 11 | |
| G5NIM1A and -B | G5 | ADH1/adh1::Ptet-caGFPb | This study |
| DSY294 | Clinical isolate, fluconazole sensitive | 34 | |
| DSY294NIM1A and -B | DSY294 | ADH1/adh1::Ptet-caGFPb | This study |
| DSY296 | Clinical isolate, fluconazole resistant, CDR1 and CDR2 overexpression | 34 | |
| DSY296NIM1A and -B | DSY296 | ADH1/adh1::Ptet-caGFPb | This study |
a
Apart from the indicated features, all strains constructed in this study are identical to their parental strains.
b
The whole cassette containing rtTA under control of the ADH1 (or OP4) promoter, the URA3 (or caSAT1) selection marker, and the indicated target gene under control of the Tet-inducible promoter was inserted into one of the ADH1 or OP4 alleles.