FIG. 5.

Inhibition of IκBα degradation and induction of p38 MAPK activation by different doses of NaSal. (A) COS-1 cells were treated for 15 min with the indicated doses of NaSal and then left unstimulated (−) or stimulated (+) for 15 min with TNF (20 ng/ml). Lysates were blotted with anti-IκBα antibody. (B) COS-1 cells were treated for 15 min with the indicated doses of NaSal alone, lysed, and blotted with anti-phospho-p38 MAPK antibody (pp38; top panel) and with anti-p38 MAPK antibody (p38; bottom panel).