Table 3.
List of key resources
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-synuclein (1:1000 dilution for Western blot) (1:250 dilution for IF) | GeneTex | 112799, RRID: AB_10618470 |
| DNAJB1 (1:1000 dilution) | Enzo Life Science | ADI-SPA-400-D, RRID: AB_2039237 |
| DNAJB2 (1:500 dilution) | GeneTex | 104464, RRID: AB_1950126 |
| DNAJB6 (1:2000 dilution) | Abcam | 198995, RRID: AB_2924896 |
| GAPDH (1:1000 dilution) | GeneTex | 100118, RRID: AB_1080976 |
| β-actin (1:2000 dilution) | Proteintech | 60008-1-Ig, RRID: AB_2289225 |
| HSPA8 (1:1000 dilution) | Novus | 120-2788, RRID: AB_2120309 |
| V5 (1:2000 dilution for Western blot) (1:250 dilution for IF) | Thermo Fisher Scientific | R960-25, RRID: AB_2556564 |
| V5 (1:100 dilution for IF) | GeneTex | 29137, RRID: AB_370778 |
| GBA (1:200 dilution for IF) | GeneTex | 101267, RRID: AB_10724323 |
| HSPA1A (1:1000 dilution) | Santa Cruz | 66048, RRID: AB_832518 |
| p-EGFR(Y1068) (1:500 dilution) | Cell Signaling Technology | 3777, RRID: AB_2096270 |
| EGFR (1:1000 dilution for Western blot) (1:100 dilution for IF) | Cell Signaling Technology | 4267, RRID: AB_2895042 |
| p-AKT(S473) (1:1000 dilution) | Cell Signaling Technology | 9271, RRID: AB_329825 |
| AKT (1:1000 dilution) | Cell Signaling Technology | 9272, RRID: AB_329827 |
| EEA1 (1:200 dilution for IF) | GeneTex | 634169, RRID: AB_2888419 |
| CD107a (LAMP-1) (1:200 dilution for IF) | Invitrogen | 14-1079-80, RRID: AB_467426 |
| SQSTM1/P62 (1:200 dilution for IF) | GeneTex | 629890, RRID: AB_2885144 |
| p-Y5 (1:250 dilution for WB) (1:500 dilution for In vitro kinase assay) | This study | N/A |
| AlexaFluorR 594 Goat Anti-mouse IgG (H + L) (1:200 dilution) | Invitrogen | A11005 |
| Alexa Fluor™ 647 Goat anti-Rabbit IgG (H + L) (1:200 dilution) | Invitrogen | A21245 |
| CyTM5-conjugated AffiniPure Goat Anti-Rabbit IgG (H + L) (1:200 dilution) | Jackson ImmunoResearch | 111-175-144 |
| AlexaFluorR 488 Chicken Anti-goat IgG (H + L) (1:200 dilution) | Invitrogen | A21467 |
| Biological sample | ||
| Human brain whole tissue lysate- adult whole normal | Novus | 820-59177, Lot# C511134 |
| Human brain whole tissue lysate- adult whole PD’s human | Novus | 820-59407, Lot# C103261 |
| Human brain whole tissue lysate- normal | GeneTex | 28771, Lot# 822400674 |
| Human brain whole tissue lysate- PD | GeneTex | 26602, Lot# 822400902 |
| Human brain whole tissue lysate- adult whole normal | Novus | 820-59177, Lot# C807591 |
| Human brain whole tissue lysate- adult whole PD’s human | Novus | 820-59407, Lot# C308158 |
| Chemicals, peptides, and recombinant proteins | ||
| DMEM | Cytiva | SH30022.02 |
| DMEM/F12 | Cytiva | SH30023.02 |
| Rotenone | Sigma-Aldrich | R8875 |
| Polybrene | Millipore | TR-1003 |
| Protein G Mag Sepharose Xtra magnetic beads | Cytiva | 28967070 |
| LuminataTM Crescendo Western HRP Substrate | Millipore | WBLUR0500 |
| DAPI | Thermo Fisher Scientifics | D1306 |
| FluoromountTM Aqueous Mounting Medium | Sigma-Aldrich | F4680 |
| T-Pro non-liposome transfection reagent II | T-Pro Biotechnology | JT97-N002M |
| LipofectamineTM LTX reagent with PLUSTM reagent | Thermo Fisher Scientific | 15338100 |
| Duolink® In Situ Wash Buffers, Fluorescence | Merck | DUO82049 |
| Duolink® In Situ PLA® Probe Anti-Mouse PLUS | Merck | DUO92001 |
| Duolink® In Situ PLA® Probe Anti-Rabbit MINUS | Merck | DUO92005 |
| Duolink® In Situ Detection Reagents Red | Merck | DUO92008 |
| NVP-AEW541 | MedChem Express | HY-50866 |
| Afatinib | MedChem Express | HY-10261 |
| Complete EDTA-free Protease Inhibitor Cocktail | Roche | 11836170001 |
| PhosSTOP Phosphatase Inhibitors | Roche | 04906837001 |
| Puromycin | Sigma-Aldrich | P8833 |
| EGFR Active human recombinant | Sigma-Aldrich | SRP0239 |
| TRIzol | Thermo Fisher Scientifics | 15596018 |
| Chloroform | Sigma-Aldrich | 32211 |
| Experimental models: Cell lines | ||
| SH-SY5Y (H.sapiens) | ATCC | CRL-2266 |
| 293 T (H.sapiens) | ATCC | CRL-3216 |
| Recombinant DNA | ||
| Please see Table S1 for a complete list of this study | N/A | N/A |
| Software and algorithms | ||
| Zen Blue2.6 | Zeiss | N/A |
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| BioRender | N/A | https://biorender.com/ |
| Other | ||
| ChemiDoc™ Imaging System | Bio-Rad | 12003153 |
| 96-well dot-blot apparatus | Bio-Rad | 170-6545 |
| Polyvinylidene difluoride membrane | Millipore | IPVH85R |
| 0.2 μm cellulose acetate membranes | Sterlitech | CA023001 |
| SulfoLink™ Immobilization Kit for Peptides | Thermo Scientific | 44999 |
| KAPA SYBR FAST | KAPA Biosystems | KK4600 |
| Maxima First Strand cDNA Synthesis Kit for RT-qPCR | Thermo Fisher Scientific | K1642 |