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. 2005 Sep 12;33(16):5156–5171. doi: 10.1093/nar/gki817

Figure 1.

Figure 1

(A) Multiple sequence alignments of the DNA binding domains of the known NtcA sequences of 17 cyanobacteria and that of the CRP of E.coli. The domain corresponds to the E and F α-helices in the crystal structure of the CRP of E.coli. Abbreviations: Synechococcus sp. WH 7803(WH7803), Prochlorococcus marinus sp. MIT 9313(MIT9313), Synechococcus sp. WH 8102(WH8102), Prochlorococcus marinus sp. CCMP1375(CCMP1375), Prochlorococcus marinus subsp. pastoris (Proch_pastoris), Prochlorococcus marinus sp. CCMP1986(MED4), Crocosphaera watsonii WH 8501(WH8501), Cyanothece sp. ATCC 51142(Cyanothece), Synechocystis sp. PCC 6803(PCC6803), Nostoc sp. PCC 7120(PCC 7120), Synechococcus sp. PCC 7002(PCC7002), Trichodesmium sp. IMS101(Trichodesmium), Plectonema boryanum (Plectonema), Synechococcus elongatus PCC 6301(PCC6301), Synechococcus sp. PCC 7942(PCC7942), Thermosynechococcus elongatus BP-1 (Thermosynechococcus), Gloeobacter violaceus PCC 7421(PCC7421), E.coli CRP (E.coliCRP). (B) Phylogenetic relationships of NtcAs of the nine genomes in our analyses. The unrooted tree was generated by the neighbor-joining method based on the multiple amino acid sequence alignments by the ClustalX program using the default settings. Scale bar, substitutions per position. (C) and (D) Logo representations of the NtcA binding sites and −10 like boxes in the training sets. Logos are generated by the Weblogo server (http://weblogo.berkeley.edu/logo.cgi).