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FISH localizing bacterial cells in leech mycteomes. (A) H. ghilianii symbionts hybridized with gammaproteobacterial probe. M, mycetome structure; D, duct. (B) P. jaegerskeoldi esophageal organ symbionts hybridized with gammaproteobacterial probe. O, esophageal organ cell; EL, esophageal lumen. No fluorescence was observed with alphaproteobacterial probes on either of these leech species. Bar, 20 μm.
