TABLE 2.
Genetic characterization of auxotrophic mutants of G. polyisoprenivorans obtained by pMA5096-mediated insertional mutagenesis
| Mutant | Insertion locus | Accession no. | Identical amino acids (%) | E value |
|---|---|---|---|---|
| A3-5-22 | argD, putative acetylornithine transaminase, Mycobacterium avium subsp. paratuberculosis k10 | AAS03681 | 108/157 (69) | 7e-46 |
| A16-03-05 | argF, putative ornithine-carbamoyltransferase, Mycobacterium tuberculosis CDC1551 | NP_336149 | 65/107 (60) | 1e-24 |
| A17-25-12 | argC, putative N-acetyl-gamma-glutamyl-phosphate reductase, Corynebacterium efficiens YS-314 | NP_738136 | 23/53 (43) | 0.18 |
| A29-56-30 | aspC, putative aspartate transaminase, Corynebacterium efficiens YS-314 | BAC19471 | 76/96 (79) | 2e-40 |
| A32-45-07 | Putative ino1, inositol-3-phosphate synthase, Nocardia farcinica IFM10152 | BAD60404 | 149/227 (66) | 4e-54 |
| A33-45-50 | Putative aminotransaminase, Streptomyces coelicolor A3(2) | NP_629136 | 21/33 (64) | 4.2 |
| A39-81-47 | aspC, putative aspartate transaminase, Nocardia farcinica IFM10152 | BAD60279 | 156/208 (75) | 8e-90 |
| B37-79-25 | Noncoding region between putative tetR regulators, Mesorhizobium loti MAFF303099 and putative ribulose-5-phosphate 4-epimerase, Rubrobacter xylanophilus DSM 9941 | ZP_00199572 | 39/83 (47) | 9e-9 |
| C8-69-39 | aspC, putative aspartate transaminase, Nocardia farcinica IFM10152 | BAD60279 | 50/65 (77) | 2e-20 |