TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or feature(s) | Reference or source |
|---|---|---|
| Y. enterocolitica strain 8081 derivatives | ||
| JB580v | ΔyenR (R− M+) | 12 |
| AJD935 | ΔyenR (R− M+) ΔaraGFB::[Φ(−lacZY)] | This study |
| AJD957 | ΔyenR (R− M+) ΔaraGFB::[Φ(pspA−lacZY)] | This study |
| AJD1022 | ΔyenR (R− M+) ΔaraGFB::[Φ(yopH−lacZY)] | This study |
| AJD1024 | ΔyenR (R− M+) ΔYE2592 | This study |
| AJD1025 | ΔyenR (R− M+) ΔYE2592 ΔaraGFB::[Φ(−lacZY)] | This study |
| Y. enterocolitica CDCa reference strains and clinical isolates | ||
| 634-83 | Serogroup O:4,32, American strain | 17 |
| 637-83 | Serogroup O:5,27, non-American strain | 17 |
| MC8 | Bio. 1, serogroup O:9, non-American strain | 17 |
| MC17 | Bio. 1, serogroup O:3, non-American strain | 17 |
| Plasmids | ||
| pFUSE | Cmr, mob+ (RP4), lacZYA+, R6K ori | 1 |
| pRE112 | Cmr, mob+ (RP4), sacB1+, R6K ori | 9 |
| pSL1180 | Apr, super polylinker, colE1 ori | Amersham |
| pSR47S | Kmr, mob+ (RP4), sacB+, R6K ori | 16 |
| pVLT33 | Kmr, tacp expression vector, RSF1010 ori | 7 |
| pWSK29 | Apr, pSC101 ori | 20 |
| pAJD509 | tacp-yscC+ in pVLT33 | This study |
| pAJD554 | tacp-ysaC+ in pVLT33 | This study |
| pAJD768 | Cmr, lacZYA+, pSC101 ori | This study |
| pAJD898 | pRE112 with ′araHG′-NotI-′araBA′ insert | This study |
| pAJD905 | pAJD898 with polylinker and lacZY from pAJD768 | This study |
| pAJD930 | pAJD905 with pspAp fragment | This study |
| pAJD952 | pAJD905 with yopHp fragment | This study |
| pAJD990 | pSR47S with ΔYE2592 insert | This study |
a
CDC, Centers for Disease Control and Prevention.