TABLE 2.
Primers
| Primer name and function | Sequence (5′ to 3′) |
|---|---|
| For amplification of ′araGH′ and ′araBA′ fragments | |
| ′araG NotI | GAAGCGGCCGCGGGAAATCGAGCAACTGTTCCG |
| araH′ SpeI | CGGACTAGTGGTTATCAGGGCATTTATCTTCAGC |
| ′araB NotI | GAAGCGGCCGCTGAACGCCAGGTTGGTCTG |
| araA′ SacI | CGGGAGCTCCTGTTTGGCTGCCGCAACG |
| For amplification of lacZY fragment with flanking rrnB terminators | |
| lacZ Up rrnBT1 | GAACGGCCGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCGGTACCTCTAGAACTATAGC |
| lacY Dwn rrnBT2 | AGAATGCGGCCGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCCGACATTGATTGCTTAAGCG |
| For colony PCR confirmation of lacZY operon fusion integrants | |
| araH check (w) | GAAATGATGTAAGCCAGACC |
| lacY check (x) | CGTCAGGTGAATGAAGTCGC |
| M13-40 (y) | GTTTTCCCAGTCACGAC |
| araA check (z) | GCATATTATCGCCGAAACGG |
| For amplification of pspA and yopH control regions | |
| pspAp Up | AGGCGATGGGCTATCAGCTC |
| pspAp Dwn | GGATCCGCACTAACTTCTGTGGAT |
| yopHp Up | GGCTCTAGATATTCGTTGCTATCACTGG |
| yopHp Dwn | GCGAGATCTAAGTTCATGCTTCCCTCC |
| For amplification of fragments flanking YE2592 | |
| ΔYE2592 Up Fwd | CGCGTCGACCCCATTTAGCGCCATCTTG |
| ΔYE2592 Up Rev | CCGGGATCCCTGCGGTTTAACTTCTTGC |
| ΔYE2592 Dwn Fwd | CCGGGATCCTGGCTAAATATCGATGGT |
| ΔYE2592 Dwn Rev | GAAGCGGCCGCTACAGGGAGCTTCACCA |